| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jessop, Autumn G (12)
|
MAUN |
36.51 |
35.25 |
-1.26 |
| 2 |
Sileo-Magliari, Sophia (10)
|
MAUDAC |
41.98 |
39.84 |
-2.14 |
| 3 |
Skalicky, Reagan A (13)
|
MAGAAC |
39.97 |
40.87 |
0.90 |
| 4 |
McGillis, Isabela L (13)
|
MACHEL |
45.86 |
44.10 |
-1.76 |
| 5 |
Tucker, Ruby C (9)
|
MACHEL |
46.88 |
45.71 |
-1.17 |
| 6 |
Cosgrove, Scarlett J (11)
|
MAGAAC |
46.06 |
45.73 |
-0.33 |
| 7 |
Abraham, Audrey (11)
|
MACHEL |
44.28 |
46.56 |
2.28 |
| 8 |
Gonnella, Ava R (10)
|
MAGAAC |
53.14 |
48.29 |
-4.85 |
| 9 |
Tooze, Grace P (9)
|
MAEAAC |
50.96 |
51.48 |
0.52 |
| 10 |
Gonnella, Lila G (9)
|
MAGAAC |
|
54.69 |
- |
|
McCrudden, Reiley M (13)
|
MAGAAC |
43.02 |
39.04 |
-3.98 |
|
Baughman, Caroline E (9)
|
MAGAAC |
44.26 |
46.09 |
1.83 |
|
Skalicky, Reese M (13)
|
MAGAAC |
39.92 |
DQ |
- |